RNA sequencing (RNA-seq) is a cost-effective and ultra-high-throughput sequencing technology that provides an efficient way to measure the whole transcription profile of a given species of interest. Camellia contains many important horticulture plants that are extensively used for making tea, edible oil, and ornamental flowers. In the last decades, increasing studies have launched to generate the transcriptome of Camellia plants at different developmental stages or under various abiotic and/or biotic stress to investigate the genetic basis of secondary metabolites that determine the tea and oil quality. We have examined almost entire available published scientific literatures describing the transcriptome sequencing experiments on tea plant and its closely relatives (as of August, 2023). These data resources freely serve the entire tea research community, and be of significance for the future genetic engineering, functional genomics and population genetic studies in Camellia.

Overview


Overview of the currently released Camellia transcriptomes (as of August 1, 2023).
Organism Cultivar Bud Leaf Flower Seed Stem Root Year Pubmed Desc
Major sequenced tissues
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Note: the tissues of Camellia plants used for transcriptome sequencing are colored with green.
Update: 2023-01-10

All assemblies

Transcriptome assembly of the representative Camellia species.
Organism Number of unigene Average length (bp) N50 (bp) GC (%) Download
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*Note: the transcriptomes from two varities of C. sinensis are used to represent the transcriptome of cultivated tea plant (Xia et al., 2017; Wei et al., 2018).

The completeness of transcriptome assembly was evaluated using plant Benchmarking Universal Single-Copy Orthologs (BUSCO) datasets (http://busco.ezlab.org/).
Update: 2023-01-10

Functional annotation

Select species:
Functional annotation of transcript is listed below:
Unigene ID Length(pb) Swiss-Prot PFAM Gene Ontology KEGG InterPros KOG COG
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EST polymorphic SSR


Polymorphic SSR Summary

Search and download specific SSR in specific Camellia species of interest
Select SSR type:        
PolySSR feasibility:
 missing rate (e.g., 0.05)
 standard deviation (e.g., 0.50)
 primer transferability (e.g., 0.99)
  (Instructions: please see CandiSSR paper for parameter details )
Results for type of Camellia EST polySSR with are listed below:
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Download all searched SSRs: 
Download primer pairs designed for each searched PolySSRs: 
Download upstream 100 bp of searched PolySSRs: 
Download downstream100 bp of searched PolySSRs: 

Transcription Factor


Camellia Transcription Factor
TF Summary

Seach and download specific TFs in specific Camellia species of interest
Select species:   
Select TF type:   
Results for All- type SSR for are listed below:
Unigene ID TF Type Families EST Sequence Protein Sequence
Download all searched SSRs:   
Instructions: The TFs in the transcriptome of each Camellia species were identified using iTAK (http://bioinfo.bti.cornell.edu/cgi-bin/itak/index.cgi) package.

Map to tea plant genome

Mapping other transcriptome assembly